Fig. 3. Migration of 4T1 cells in different amounts of RAW 264.7 macrophage-conditioned media: non-treated (CM-NT) or PBP-treated (CM-PBP). Cells were cultured in CM-NT or CM-PBP for 24 h. Migration patterns of 4T1 cells were assessed using the colorimetric transwell cell migration assay. The level of migration of CM-treated cells was expressed as a fold change relative to equivalent control cells incubated in complete DMEM medium containing 10, 25, 50 or 75% of 1% FBS/DMEM medium. Data are from three independent experiments and presented as the mean ± SEM. Asterisks show significant differences between the cells cultured in CM-NT compared with the CM-PBP-treated cells (***P<0.001). Hash marks denote differences in the migration level between cells cultured in the suitable conditioned medium and control cells (horizontal line) (^{# # #}P<0.001; ^{# #}P<0.01).